Our latest paper is published in Nature Communications!

Excited to share our paper tracking #flu vRNP and Rab11A in live cells reveals alteration of dynein motors on Rab11A bound by #flu vRNP. Using #diSPIM we built @PittHealthSci with collaborator Hari Shroff @NIBIBgov and proteomics work with @WeitzmanLab 1/9 https://t.co/emPHAj24E2

— S_Lakdawala (@Lakdawala_Lab) January 7, 2020

Two color #diSPIM to track host Rab11A GTPases (GFP) and influenza viral RNP (PA::mRuby) in a single infected A549 cell revealed that Rab11A colocated with vRNP moved slower and more arrested than unbound Rab11A GTPases This was true during flu A and B infection. 3/9 pic.twitter.com/ihElmPO5a8

— S_Lakdawala (@Lakdawala_Lab) January 7, 2020

Rab11A proteomics in uninfected and influenza virus infected A549 cells revealed a reduction of dynein levels associated with Rab11A during virus infection, which alters the motor stoichiometry and transport dynamics. 5/9

— S_Lakdawala (@Lakdawala_Lab) January 7, 2020

But (surprise!!) influenza vRNP movement (PA-tagged GFP) was mostly unaltered. 7/9 pic.twitter.com/iRbrEVgcNm

— S_Lakdawala (@Lakdawala_Lab) January 7, 2020

This paper was 4 years in the making and I was so lucky to recruit Amar Bhagwat, Eric Nturibi and @SageVle to help get this story together! Below is a picture of Amar and Eric the first time we were able to capture movement GFP-tagged Rab11A on our diSPIM. 9/9 pic.twitter.com/R7CzPk9O0D

— S_Lakdawala (@Lakdawala_Lab) January 7, 2020